Table 1.
Primer sequences, position, product length, and CpG unit used for MassArray quantitative methylation analysis
| Regimen | Primer | Position (bp) | Product Length (bp) | CpG units |
|---|---|---|---|---|
| #1 | AGGATAATGTTGTTGTTGAGGTAGG (F)1 | -1690∼-1277 | 414 | 12 |
| CTAAATCCCAAATACTCCCAAATTC (R)2 | ||||
| #2 | GGTTGGGTTGATTTTAGGTTTTAGT (F)1 | -1190∼-701 | 490 | 15 |
| CCTCCTACTATCCCCCTAATTACAC (R)2 | ||||
| #3 | ATTAGGGGGATAGTAGGAGGAGTTT (F)1 | -720∼-258 | 563 | 14 |
| CAAATTTCAAAAAAATAATTCCCTC (R)2 | ||||
| #4 | GAGGAGGGAATTATTTTTTTGAAAT (F)1 | -285∼59 | 345 | 12 |
| AACCAATACCCACCAACAACTC (R)2 |
Note:1tag: aggaagagag and 2tag: cagtaatacgactcactatagggagaaggct were added.