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. 2019 Jan 1;15(2):277–286. doi: 10.7150/ijbs.30348

Figure 5.

Figure 5

Investigation the PI3K/AKT signaling pathway in the anti-apoptotic effect of ICA in vitro. (A) Observation of cell morphology (Scale bars = 50 µm). (B) Western blot assays of ER stress-induced apoptosis. (C) Immunofluorescence staining (Scale bars = 50 µm). (D, E) Cell viability after the treatment with TG and with or without ICA. (F) Quantitative analysis of western blot assays. (G) Cell viability after treatment with or without TG, ICA and LY294002. The primary cultured spinal cord neurons were cultured with great growth state, appropriate density and high purity. The cell viability decreased by half at 2 μM TG. The cell viability was significantly increased by ICA pretreatment (10 - 20 μM). ICA decreased the expression of apoptotic proteins and enhanced cell viability after SCI. However, LY294002 administration significantly reversed this beneficial effect.**P < 0.05 compared with control;*P < 0.05 compared with TG or SCI group;#P < 0.05 compared with ICA group.