MET promotes angiogenesis in random skin flaps. (A&C) IHC for VEGF and Cadherin 5 expressions in the ischemic flaps of the Control and MET groups (original magnification × 200; scale bar, 50μm). (B&D) The optical density values of VEGF and Cadherin 5 were quantified and analyzed in each group. (E-G) Western blotting for VEGF, MMP9, and Cadherin 5 expressions in the ischemic flaps of the Control and MET groups. The gels have been run under the same experimental conditions, and cropped blots are used here. (H-J) Optical density values of VEGF, MMP9, and Cadherin 5 were quantified and analyzed in each group. Values are shown as means± SEM, n= 6 per group. *p< 0.05 and **p< 0.01, vs. Control group.