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. 2019 Jan 1;15(2):325–340. doi: 10.7150/ijbs.29009

Figure 6.

Figure 6

Inhibition of autophagy reverses the effects of MET on angiogenesis, apoptosis and oxidative stress. (A) Autophagosomes (red) in cells in Area II of flaps in the MET and MET+3MA groups by Immunofluorescence staining for LC3II (scale bar, 25μm). (B&D) The expressions of autophagy-related protein VPS34, p62, LC3II, Beclin1, and CTSD; angiogenesis-related protein VEGF, Cadherin 5, and MMP9; oxidative stress-related protein SOD1, HO1, and eNOS and apoptosis-related protein Bax, CYC, and CASP3 in each group, were assessed by Western blotting. The gels have been run under the same experimental conditions, and cropped blots are used here. (C&E) Optical density values of VPS34, p62, LC3II, Beclin1, CTSD, VEGF, Cadherin 5, MMP9, SOD1, HO1, eNOS, Bax, CYC, and CASP3 expressions in the two groups. Values are expressed as means± SEM, n= 6 per group. #p< 0.05 and ##p< 0.01, vs. MET group.