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. 2019 Jan 1;15(2):369–385. doi: 10.7150/ijbs.28422

Figure 2.

Figure 2

Lentivirus-directed ING4 overexpression or knockdown in human HCC cells. A. Western blot analysis of ING4 in HCC cells. The lysates of MHCC97H and MHCC97L HCC cells and HL-7702 normal liver cells were immunoblotted with anti-ING4 or anti-β-actin (a loading control) antibody. The representative pictures of Western blot were shown. *, p<0.05 compared with HL-7702; *, p<0.05 compared with MHCC97L, one-way repeated measures ANOVA, n=6 replicates per sample. B. Flow cytometric analysis of GFP expression. C. Western blot analysis of lentivirus-mediated ING4 overexpression or knockdown. The lysates of MHCC97H-ING4 vs MHCC97H-mock and MHCC97L-shING4 vs MHCC97L-shcontrol HCC cells were immunoblotted with anti-ING4 or anti-β-actin (a loading control) antibody. The representative pictures of Western blot were shown. MHCC97H: *, p<0.05 compared with MHCC97H-mock; MHCC97L: *, p<0.05 compared with MHCC97L-shcontrol, Student t test, n=6 replicates per sample. The expression level of ING4 in these Western blot assays was normalized to β-actin (ING4/β-actin) and then expressed as a ratio or fold of respective control, with 1 being the value for HL-7702, MHCC97H-mock or MHCC97L-shcontrol control. Data shown were representative of three independent experiments.