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. 2019 Jan 1;15(2):404–415. doi: 10.7150/ijbs.27912

Figure 2.

Figure 2

Effect of LH on relative abundance of mRNA of (A) MT1, (B)MT2, (C)CYP17A1 and melatonin-induced expression of (D-I) STAR, CYP11A1, HSD3B1, CYP17A1, LHR in TI. Small follicles were treated with different doses of LH (0, 0.1IU, 1IU) or with 10ng/mL melatonin and 0.1 IU/mL LH alone or in combination for 48h. Total RNA was extracted from TI. The mRNA expression of MT1, MT2, STAR, CYP11A1, HSD3B1, CYP17A1 and LHR was measured by RT-qPCR. GAPDH expression was used as a standard. TI lysates were subjected to SDS-PAGE/immunoblotting for STAR, CYP11A1, HSD3B1, CYP17A1, LHR and GAPDH analysis. The relative density ratio was calculated using a control group value of one. The results are the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01. n.s.: not significant.