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. 2019 Jan 1;15(3):522–532. doi: 10.7150/ijbs.30572

Figure 5.

Figure 5

Enzalutamide resensitizes palbociclib-resistant cells to palbociclib. A. MCF-7pR cells were treated for 48 hours with 20 µM of enzalutamide, 4 µM of palbociclib or both. Flow cytometry was used to identify the distribution of cell cycle compared to the cells with no treatment. B. MCF-7pR cells were treated with the combination of palbociclib and enzalutamide. The dose radio of palbociclib and enzalutamide was 1:1. Cell viability was investigated by Cell Counting kit-8 assay. CI vs. Fa plot (combination index vs. fraction affected) for cell viability data are shown. The CI values were calculated by Compusyn software. CI values below 1 are considered to have a synergistic interaction. Data represented the mean of the three independent experiments. C. MCF-7pR cells were treated with 10-160 µM enzalutamide combined with a constant dose of palbociclib (2, 4, 8, 16 µM) for 48 hours. CI values calculated by Compusyn software are shown. D. MCF-7pR cells were treated with palbociclib and enzalutamide alone or in combination for 48 hours and cell proliferation was determined by clone formation assay. Data represents the mean of the three independent experiments, ***P < 0.001. E. MCF-7pR cells were treated with 20 µM of enzalutamide with or without 4 µM of palbociclib for 48 hours. After treatment of drugs, western blot was performed using anti-pRb (s795), anti-Rb, anti-E2F1, anti-cyclin A2 and anti-cyclin E2.