Figure 6.

The synergistic effect of enzalutamide and palbociclib on palbociclib-resistant cells in vivo. Mice bearing MCF-7pR xenografts with a tumor volume of 100±50mm³ (6 for each group) were dosed with palbociclib (100mg/kg/d, p.o.) twice a week or enzalutamide (50 mg/kg/d, p.o.) five times a week or both. A. Tumor volumes were evaluated once a week and calculated by the formula:(S2 × L) /2. S: short diameter; L: long diameter, *P < 0.05. B. Body weight data for MCF-7pR xenograft mouse. C-D. Tumors from mice treated with each of the treatment arms were extracted and stained with routine H&E staining (200×). The xenograft tumors were subjected to immunohistochemical analysis of treated tumors for Ki67 and AR. Expression ratio of ki67 and AR in each of the treatment arms are shown, ***P < 0.001.