Skip to main content
. 2019 Jan 24;15(3):701–713. doi: 10.7150/ijbs.29323

Figure 5.

Figure 5

Effects of HG on Sirt6 expression in cultured podocytes. (A) Representative Western blots of Sirt6 expression in cultured podocytes stimulated with different concentrations of glucose for 24 h and quantitation of these results (n=4). *P< 0.05 compared with cells treated with NG. (B) Representative Western blots of Sirt6 expression in 30 mM HG-treated podocytes at various times points and quantitation of these results (n=4). *P< 0.05 compared with cells treated with HG for 0 h. (C) Representative Western blots of Sirt6 expression and quantitation of these results in podocytes cultured with different medium (n=4). *P< 0.05 compared with cells in the NG or MA group. (D) Immunofluorescence results of Sirt6 in cultured podocytes in each group(original magnification, ×400). NG=5 mM glucose for 24 h, MA=5 mM glucose+25 mM mannitol for 24 h, HG=30 mM glucose for 24 h.