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. 2019 Jan 24;15(3):647–656. doi: 10.7150/ijbs.31293

Figure 5.

Figure 5

Target genes regulated by FBXO22. (A, B) Western blot analysis was used to detect the relative protein levels of N-cadherin, MMP‑9, TIMP1 and VEGFA in FBXO22 low expression group and control group for both 786-O and ACHN cell lines. GAPDH was used as a loading control. (C, D) The activity of MMP-9 were evaluated by Gelatin zymography. (E, F) ELISA for the secretion of VEGF in 786-O and ACHN cells Transduced with control siRNA (siCtrl) or small interfering RNAs (siRNAs) targeting FBXO22. Data are presented as the mean ± SD for three independent experiments, *P < 0.05, **P<0.01,***, P < 0.001.