Fig 2.

Expression profile analysis of RRM2 knockdown in U87 glioma cells by RNA-Seq and bioinformatics. A Scatter plot for differentially expressed genes between RRM2 knock-down group and control. Grey points indicate the non-significant differentially expressed gene whose GFOLD value is zero. Colored points were significant differentially expressed genes (GFOLD≠0). With an additional strict cutoff of 1.3-fold change, 1234 and 2276 genes were determined as highly up- or down-regulated genes, respectively. B Differences in biological process scored by GSEA between the knock-down group and the control group. Shown are normalized enrichment scores (NES) indicating whether the GO gene sets is mobilized as a whole by RRM2 knock-down, either toward down-regulation (NES < 0) or toward up-regulation (NES > 0). C and D Gene ontology and KEGG pathway enrichment analysis which shows the distribution of terms exhibiting statistically significant differences (Fisher exact test, p-values were adjusted for multiple testing using the Benjamini-Hochberg procedure which were represented with the color scale). Only genes with at least 1.3-fold change were included to perform enrichment analysis.