Fig 4.

Interfering RRM2 inhibits the proliferation of cancer cells. A and B Western blot analysis showed that cyclin A, cyclin B1, and cyclin D1 were down-regulated in RRM2-transfected U87 cells. data expressed as mean ± SD of three independent experiments, # P<0.001 and Ф P<0.001, versus shNC. C RRM2 depletion significantly increased the proportion of U87 cells in G2 and M phase using flow cytometry. D and E Cell proliferation was detected by CCK-8 assay in U87 and LN229 cells; data expressed as mean ± SD of three independent experiments, # P< 0.001 and Ф P< 0.001, versus shNC. F and G Silencing of RRM2 inhibited the growth of U87 cells analyzed by colony-forming assay. The colonies were calculated. data expressed as mean ± SD of three independent experiments, # P< 0.001 and Ф P< 0.001, versus shNC.