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. 2019 Jan 1;15(3):507–521. doi: 10.7150/ijbs.30575

Figure 3.

Figure 3

ARPE-19 cell monolayer transit into fibroblastoid phenotype under EMT induced by TGF-β2. (a) Images of ARPE-19 cell monolayer either untreated (control) or treated with TGF-β2 (10 ng/ml) for 24 h. scale bar, 100 μm. (b) Western blot analysis of epithelial marker claudin-1 and mesenchymal marker N-cadherin, Vimentin, αSMA, under different time points treatment of TGF-β2 (10 ng/ml). Quantification of protein expression were shown as the means±S.D. **P<0.01 vs 0 h. (c) Immunocytochemistry micrographs showed loss of claudin-1 (red) on cell membrane and increased N-cadherin (green) after TGF-β2 (10 ng/ml) treatment for 24 h. Nuclei were stained with DAPI (blue); scale bar, 50 μm.