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. 2019 Jan 1;15(3):507–521. doi: 10.7150/ijbs.30575

Figure 6.

Figure 6

Autophagy maintain epithelia phenotype in RPE. (a) Western blot analysis of p62, LC3, claudin-1 and N-cadherin in Atg7-knockdown cells by overexpressing Myc-Atg7 NT (nontargetable mutant). Quantification of protein expression were shown as the means±S.D. *P<0.05, **P<0.01. (b) Western blot analysis of Atg7, p62, LC3, claudin-1 and N-cadherin under different time points of serum-starvation. Quantification of protein expression were shown as the means±S.D. *P<0.05, **P<0.01 vs 0 h. (c) Immunocytochemistry staining showed increased claudin-1 and weaken N-cadherin on cell membrane after 48 h serum-starvation; scale bar, 50 μm.