Figure 3.

Robo1 Expression in the Nerve Bridge

(A) Robo1 staining on a longitudinal nerve bridge section at 7-days post-injury from a PLP-GFP mouse showing that Robo1 is highly expressed in migrating cells of the nerve bridge.

(B) Higher magnification image showing migrating GFP+ Schwann cells inside the nerve bridge.

(C) Higher magnification image showing Robo1 positive cells inside the nerve bridge.

(D) Merged image showing Schwann cells inside the nerve bridge express Robo1.

(E) Vimentin staining showing migrating fibroblasts in the nerve bridge.

(F) Robo1 staining showing Robo1 positive cells inside the nerve bridge.

(G) Merged image showing migrating fibroblasts in the nerve bridge express Robo1.

(H) Robo1 staining in the distal nerve stump of PLP-GFP mice.

(I) Merged image showing Schwann cells in the distal nerve stump express high levels of Robo1.

(J) Robo1 staining in cultured rat Schwann cells.

Scale bar in (A) represents 200 μm, in (B)–(G) represents 20 μm, and in (H)–(J) represents 10 μm.

Several z series were captured on a Zeiss LSM510 confocal microscope in (A), covering the entire field of interest. The individual series were then flattened into a single image for each location and combined into one image using Adobe Photoshop software (Adobe Systems).