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. 2019 Feb 5;26(6):1458–1472.e4. doi: 10.1016/j.celrep.2018.12.081

Figure 6.

Figure 6

Slit3-Robo1 Signaling Controls the Trajectory and the Distance of Schwann Cell Migration Inside the Nerve Bridge

(A–C) Fourteen days after transection injury, in control mice, GFP+ Schwann cell cords connect the proximal and distal nerve stumps. In Slit3+/−:Robo1+/− mice (B and C), a large population of Schwann cells leave the nerve bridge from both the proximal nerve end (indicated by white arrows) and the distal nerve end (indicated by yellow arrows). Bridge length in (A) and (C) is 2.1 mm and in (B) is 1.1 mm.

(D and E) The distance of leading Schwann cells inside the nerve bridge from the proximal nerve end (D) and from the distal nerve end (E) 5 days after nerve transection, n = 3. ∗∗p < 0.01 compared with controls.

(F–J) Schwann cell migration in control (F), Slit2+/− (G), Slit3+/− (H), Robo1+/− (Ι), and Slit3+/−:Robo1+/− (J) mice 5 days after transection injury.

Scale bar in (A)–(C) and (F)–(J) represents 200 μm.

Several z series were captured on a Zeiss LSM510 confocal microscope in (A) –(C) and (F) –(J), covering the entire field of interest. The individual series were then flattened into a single image for each location and combined into one image using Adobe Photoshop software (Adobe Systems).