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. 2019 Feb 1;10:48. doi: 10.3389/fphar.2019.00048

FIGURE 7.

FIGURE 7

CBS improved hepatic bile acid homeostasis is abrogated by FXR antagonist GS. (A) Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). (B) Serum levels of liver total bile acids (TBA). (C) Total and nuclear protein levels of hepatic FXR were determined by Western blot analysis and normalized to β-actin and Lamin B. (D) mRNA expression of hepatic Shp, Bsep and Cyp7a1 were evaluated by real-time PCR and normalized to β-actin. (E) Total and nuclear protein levels of intestinal FXR were determined by Western blot analysis and normalized to β-actin and Lamin B. (F) mRNA expression of intestinal Fgf15 was determined by real-time PCR and normalized to β-actin. (G) Representative images of hematoxylin and eosin straining. Data are presented as the mean ± SD (n = 6). Significant differences compared with the non-cholestatic group, ∗p < 0.05; ∗∗p < 0.01; compared with the 17α-ethinylestradiol (EE) group, #p < 0.05; ##p < 0.01.