Fig. 3.

M1 is less potent than tacrolimus as an immunosuppressant and antifungal agent. (A) Immunosuppressant activities of tacrolimus and M1 were examined in PBMCs by measuring cell proliferation after treatment with a T-lymphocyte mitogen in the presence of tacrolimus or M1. (B) Antifungal activities of tacrolimus and M1 were examined using Malassezia sympodialis. The yeast was inoculated on a modified Dixon agar plate. After 1 hour incubation, an aliquot of tacrolimus or M1 at different concentrations was placed on the plate, as shown in the left panel, and incubated at 37°C for 2 days.