TABLE 1.
Screening gut bacteria for tacrolimus conversion to M1 in YCFA culture
| Order | Bacterium | OD600 | M1 Production Detected |
|---|---|---|---|
| Bifidobacteriales | Bifidobacterium longum | 1.8 | No |
| Bacteroidales | Bacteroides cellulosilyticus | 0.6 | Yesa |
| Bacteroides finegoldii | 3.4 | Yesa | |
| Bacteroides ovatus | 4.2 | Yesa | |
| Parabacteroides merdae | 2.7 | Yesa | |
| Parabacteroides johnsonii | 3.6 | Yesa | |
| Parabacteroides goldsteinii | 3.3 | Yesa | |
| Clostridiales | Ruminococcaceae sp. | 0.5 | Yes |
| Clostridium innocuum | 3.4 | Yes | |
| Anaerostipes sp. | 2.7 | Yes | |
| Dorea formicigenerans | 2.4 | Yes | |
| Clostridium clostridioforme | 3.0 | Yes | |
| Clostridium hathewayi | 2.6 | Yes | |
| Blautia sp. | 4.7 | Yes | |
| Clostridium aldenense | 1.4 | Yes | |
| Clostridium symbiosum | 2.5 | Yes | |
| Clostridium citroniae | 1.7 | Yes | |
| Coprococcus sp. | 2.4 | Yes | |
| Clostridium bolteae | 3.6 | Yes | |
| Clostridium cadaveris | 1.4 | Yes | |
| Ruminococcus gnavus | 3.4 | Yes | |
| Erysipelotrichales | Erysipelotrichaceae sp. | 3.8 | Yes |
a
M1 production observed only when using sensitive HPLC-MS/MS for detection.