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. 2019 Mar;368(3):338–352. doi: 10.1124/jpet.118.253955

Fig. 5.

Fig. 5.

Effects of altenusin on the release of nitrite and TNF-α in primary rat microglia (A and B). For estimation of nitrite and TNF-α release, cells were pretreated with altenusin (10–50 µM) for 30 minutes followed by stimulation with LPS (20 ng/ml) for the next 24 hours. At the end of incubation, cell supernatants were collected and centrifuged and levels of nitrite (A), and TNF-α (B) was quantified by using Griess reagent assay and rat specific ELISA assay, respectively. For miRNA expression of miR-155 (C) and miR-146 (D), cells were pretreated with altenusin (50–100 µM) and GW4869 (5–10 µM) for 30 minutes, followed by LPS stimulation for 4 hours. Altenusin (100 µM) and GW4869 (10 µM) significantly decreased the miR-155 (C), but not miR-146 (D), expression in LPS-stimulated BV2 cells. Statistical analyses were carried out by using one-way ANOVA with post hoc Tukey’s test (multiple comparisons). Results are expressed as means ± S.E. of at least three independent experiments. **P < 0.01; ***P < 0.001 in comparison with LPS; ###P < 0.001 in comparison with control.