The effect of HSD1169 on the activity of 15 nM FLT3, 100 nM FLT3 (ITD), 0.5 nM FLT3 (D835Y) and 20 nM TOPK in the presence of 100 μM ATP (done at Reaction Biology Corporation). Enzyme activity in the presence of HSD1169 was normalized to the DMSO control and data fitted to a nonlinear regression using GraphPad Prism (GraphPad, CA, USA). Error bar represent standard error of the mean of duplicate reactions.
ITD: Internal tandem duplication; TOPK: T-LAK cell-originated protein kinase.