Figure 5. Modulating METTL16 Activity Alters MAT2A mRNA Levels.

(A) Schematic of the reporter construct used in cell-based assays.

(B) Representative northern blot of β-globin (MAT2A mRNA) in cells transfected with the reporter (A) and wild-type or mutant FLAG-METTL16, from cells grown over a range of methionine concentrations. Images were cropped from the same gel at the same exposure. GAPDH northern blot is shown below as a loading control.

(C) Quantification of relative mRNA normalized to GAPDH, expressed relative to WT at 1,000 μM methionine from three replicates of (B). Data are shown as the mean ± SD. p values are indicated with asterisks over bars that were compared using unpaired Student’s t test: *p < 0.05, **p < 0.01, ***p < 0.001.

(D) Representative northern blot of β-globin (MAT2A mRNA) in cells transfected with the reporter (A) and wild-type or mutant FLAG-METTL16, from cells grown over a range of methionine concentrations. Images were cropped from the same gel at the same exposure. GAPDH northern blot is shown below as a loading control.

(E) Quantification of relative mRNA normalized to GAPDH, expressed relative to WT at 1,000 μM methionine from three replicates of (D). Data are shown as the mean ± SD. p values are indicated with asterisks over bars that were compared using unpaired Student’s t test: *p < 0.05, **p < 0.01, ***p < 0.001.

See also Figure S5.