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. Author manuscript; available in PMC: 2019 Feb 8.
Published in final edited form as: J Alzheimers Dis. 2019;67(1):137–147. doi: 10.3233/JAD-180858

Fig. 4.

Fig. 4.

Propofol significantly increased acidity of lysosome in L286V cells by over activation of InsP3 (InsP3R) or ryanodine (RYR) receptors. A) Representative pictures of lysosome acidity (red staining) determined by Lysotracker. With or without propofol treatment (1 versus 100 μM) and in the presence or absence of antagonists for InsP3Rs (xestospongin C, Xc) or RYRs (dantrolene, Dan). Scale bar = 20 μm. B) Propofol only at non-clinical high concentrations (100 μM) increased acidity, more significantly in L286V cells, which were abolished by Xc or Dan. All data are expressed as the mean ± SEM from at least three separate experiments (N ≥ 3) of at least twenty individual cells and analyzed by two-way ANOVA followed by Tukey multiple comparison tests. *p < 0.01 and **p < 0.001.