Fig. 5.
A comparison of MUSE image quality with various stromal/cytoplasmic stains. The images show examples of fresh tissues stained with (a) eosin and (b) ATTO 655 NHS ester. (c) With fresh, hydrated specimens, the leakage of eosin, which is poorly bound, results in a pooling effect around the edge of the specimen, and also reduces (e) and (g) image contrast of microstructures. ATTO 655 NHS ester does not exhibit leaking at the (d) tissue edges and provides improved (f) and (h) microstructural contrast. Scale bar: (a) and (b) 1 mm and (c)–(h) .