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. Author manuscript; available in PMC: 2020 Jan 10.
Published in final edited form as: Cell. 2019 Jan 10;176(1-2):113–126.e15. doi: 10.1016/j.cell.2018.12.002

Figure 2. PMN activation confers increased expression of enzymatically active, α−1 AT resistant NE.

Figure 2.

(A) NE activity of activated and quiescent exosomes measured at 5-minute intervals for production of (BODIPY FL) labelled fluorescent elastin fragments from self-quenching BODIPY FL-conjugated bovine neck ligament elastin. (B) NE activity of activated and quiescent exosomes against specific NE peptidomimetic substrate MeOSucAAPVpNA, by pNA generation. (C) 20ng purified NE coincubated with Human NE Inhibitor II, the endogenous NE inhibitor α−1 AT or PBS control, and NE activity by pNA generation measured as in B. (D) NE activity of an equipotent number of activated exosomes (1 × 109) and 20ng of purified NE measured with Human NE Inhibitor II, α1-AT, or PBS control as in B. Single experiment shown in C. and D. displayed separately for clarity. Data shown as mean +/− SEM. See also figure S2.