Fig. 5.

—Evolutionary dynamics of copy number of the gene SEUSS in J. sinuosa. (A) Lineage-specific expansion of SEUSS in J. sinuosa. The ten duplicated copies were labeled according to their relative position order along the located scaffolds. Among them, seven copies were annotated by the MAKER pipeline. (B) Validation that there are multiple copies of SEUSS using read depth of Illumina DNA-seq reads which is at least equal or above the genomic background and adjacent single-copy genes. Each dot represents read depth from a 1 kb sliding window. The red dots indicate the windows that overlapped with SEUSS genes, whereas the blue dots indicate the windows that overlapped with other single-copy genes. The gray dots are the windows that contain other (intergenic) regions.