Figure 5. Kaplan–Meier metastasis-free survival analysis of TGFβ-EMT and TGFβ-EMT_N signatures and CDH1 and LAG3 immunohistochemistry staining in formalin-fixed paraffin-embedded samples.

TGFβ-EMT_N high, CDH1 high, and LAG3 high are colored as red; TGFβ-EMT_N low, CDH1 low, and LAG3 low are colored as blue. (A) Samples with high TGFβ-EMT signature score exhibit significantly worse metastasis-free survival. (B) Magnitude of the difference between high and low groups is similar within the early-stage subset of the cohort. Immunohistochemistry staining for CDH1 (C) and LAG3 (D) are not associated as strongly with MFS as the TGFβ-EMT or TGFβ-EMT_N signatures, as they exhibit higher P-values and lesser separation between high and low curves than in (A) and (B).