Table 4. Association of immunohistochemistry staining with survival in formalin fixed paraffin-embedded samples.
| Overall survival | Metastasis-free survival | |||
|---|---|---|---|---|
| 3 year | 5 year | 3 year | 5 year | |
| TGFβ-EMT | 4.62 × 10−4 | 5.69 × 10−5 | 1.37 × 10−3 | 1.59 × 10−4 |
| TGFβ-EMTN | 5.82 × 10−4 | 8.78 × 10−4 | 5.91 × 10−4 | 1.49 × 10−3 |
| A2A | 0.736 | 0.619 | 0.890 | 0.559 |
| BTLA | 0.486 | 0.745 | 0.439 | 0.865 |
| CDH1 | 0.112 | 0.018 | 0.091 | 0.023 |
| CTLA4 | 0.371 | 0.100 | 0.424 | 0.195 |
| INOS | 0.481 | 0.973 | 0.593 | 0.697 |
| LAG3 | 0.375 | 0.397 | 0.050 | 0.021 |
| PDL-1 | 0.792 | 0.839 | 0.599 | 0.415 |
| PLAUR | 0.346 | 0.189 | 0.877 | 0.812 |
| SMAD7 | 0.409 | 0.572 | 0.161 | 0.242 |
| TIM3 | 0.219 | 0.316 | 0.388 | 0.532 |
Kaplan–Meier log-rank test P-values are reported for immunohistochemistry staining of 10 genes. MFS ratios at 3- and 5- yrs. represent the proportion of patients that have not suffered an event (metastasis or death). The TGFβ-EMTN signature was significantly associated with both OS and MFS at both 3 and 5 years. Positive E-cadherin 1 (CDH1) staining is significantly associated with OS (P = 0.018) and MFS (P = 0.023) only at 5 years. Interestingly, lack of LAG3 signal is significantly associated with MFS 5 years (P = 0.021), and borderline significant at 3 years (P = 0.050).