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. 2019 Jan 25;10(8):825–846. doi: 10.18632/oncotarget.26582

Figure 10. Induction of cell death in U87MG spheroid cultures following treatments with CBD, ATM inhibitor (ATMi), and γ-irradiation (10 Gy), alone or in combination.

Figure 10

(A) Phase-contrast images of U87MG neurospheres 48 h after treatment with 0.1% DMSO (control) or CBD (10 μM), alone or in combination with γ-irradiation (10 Gy) or γ-irradiation + ATMi (2 μM). (B) Cell viability assay was performed for U87MG neurosphere cultures 48 h after indicated treatments. Pooled results of four independent experiments 48 h after treatments are shown. Error bars represent means ±S.D. (p < 0.05, Student's t-test). Arrows and stars indicate significant differences in cell viability after specified treatments. (C) U87MG neurospheres from suspension cultures (72 h after treatment) were attached to coverslips and immunostained with anti-CD133 mAb (green).