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. 2019 Jan 25;10(8):825–846. doi: 10.18632/oncotarget.26582

Figure 2. CBD, alone or in combination with ATMi and γ-irradiation (10 Gy) decreased the viability of U87MG cells.

Figure 2

(A) Effects of CBD (10 μM), ATMi (2 μM) and γ-irradiation (10 Gy) on functional activity of mitochondria in U87MG cells detected 6 h after treatment using Mitotracker (green) incubation and confocal microscopy. Additionally, cells were stained with anti-α-Tubulin mAb (red) and DNA-specific DAPI (blue). Bar = 20 μm. (B) Relative levels of Mitotracker-induced green fluorescence after indicated treatments of U87MG cells. Pooled results of three independent experiments 48 h after treatments are shown. Error bars represent means ±S.D. (p < 0.05, Student's t-test). (C-E) Effects of CBD (5-40 μM) and ATMi (1-5 μM), alone or in combination with γ-irradiation (10 Gy) on U87MG cell viability using the determination of intracellular ATP via ATP-dependent luminescence intensity 48 h after treatment. Combinations of CBD (5-20 μM), ATMi (2 μM) and γ-irradiation (10 Gy) were used in the panel E. Stars, arrows, circles, and squares indicate significant differences in the cell viability after specified treatments.