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. 2019 Jan 25;10(8):825–846. doi: 10.18632/oncotarget.26582

Figure 5. Changes in the pattern of cell signaling proteins of T98G human GBM cells and regulation of cell death after treatment with ATMi alone or in combination with CBD and γ-irradiation.

Figure 5

(A) DNA DSB were induced in the nuclei of treated T98G cells and detected by immunostaining with anti-γH2AX Ab (green foci) and DAPI staining of DNA (blue). (B) Western blot analysis of indicated signaling proteins from T98G cells was performed 4 h after treatments with CBD (10 μM), the ATMi KU60019 (2 μM), alone or in combination, without or with γ-irradiation (10 Gy). CBD, ATMi, and DMSO (a vehicle) were added to the cell cultures 30 min before irradiation. (C and D) Apoptotic levels in T98G cells were detected using Annexin-V-FITC and PI staining, as described in Figure 3A. A typical result (C) and the pooled results of four independent experiments are presented (D). Error bars represent means ± S.D. (p < 0.05, Student's t-test). The arrows and circles indicate significant differences in cell death levels between control cells and cells after specified treatments.