Fig. 3.
Glycolysis is altered in granule cells of Oxr1 knockout mice. a The extracellular acidification rate (ECAR) using the Seahorse glycolysis stress test assay; the timing of the compounds added to the assay medium are indicated (glucose to feed glycolysis, followed by oligomycin to inhibit mitochondrial ATP synthase and 2-deoxy-D-glucose (2-DG) to inhibit glycolysis) and the various parameters that can be calculated are represented by arrows (non-glycolytic acidification (i), glycolysis (ii), glycolytic capacity (iii), and glycolytic reserve (iv)). b–f ECAR assay data from primary CGCs from Oxr1d/d and Oxr1+/+ mice in untreated (b–d) or arsenite-treated (d–f) conditions (N = 4 animals per group for all panels). For comparative purposes, panel d represents the data from panels c and f. Panels b, e: two-way ANOVA, panels c, d, f: t test; *p < 0.05, **p < 0.01