Fig. 6.

Oxr1 binding affinity to Gpi1 is influenced by disease associated-mutations. a–d Co-immunoprecipitations using an anti-MYC antibody and direct protein extracts in cells co-expressing MYC-tagged Gpi1 (wild-type (WT) or mutant constructs) with HA-tagged Oxr1-FL (panels a–b N = 3 independent repeats) or Oxr1-C (panels c–d N = 4 independent repeats). e–f Predictions of the monomeric (e), dimeric (e), and tetrameric (f) forms of mouse Gpi1 interacting with the TLDc domain. g Representation of the Gpi1 dimer with the positions of the key residues mutated and investigated highlighted. Panels b, d: one-way ANOVA; *p < 0.05