Table 2.
Primer | Sequence |
---|---|
GAPDH forward | 5′-GGCATGGACTGTGGTCATGA-3′ |
GAPDH reverse | 5′-TTCACCATGGAGAAGGC -3′ |
HBO1 forward | 5′-AAAGAGACAGGACCTGATTG-3′ |
HBO1 reverse | 5′-GCAACAGAGAAATGACAGAC-3′ |
USP25 forward | 5′-AATGTGATTGATCTCACTGG-3′ |
USP25 reverse | 5′TCATCAGTTATTCCAGTCTCC-3′ |
IL-1β forward | 5′-CTAAACAGATGAAGTGCTCC-3′ |
IL-1β reverse | 5′-GGTCATTCTCCTGGAAGG-3′ |
IL-6 forward | 5′-GCAGAAAAAGCAAAGAATC-3′ |
IL-6 reverse | 5′-CTACATTTGCCGAAGAGC-3′ |
IL- 10 forward | 5′-GCCTTTAATAAGCTCCAAGAG-3′ |
IL- 10 reverse | 5′-ATCTTCATTGTCATGTAGGC-3′ |
DsiRNA | |
USP25-DsiRNA-1 | 5′-AAGAGAAUCCCAAAGUACUUGAATA-3′ 3′-AGUUCUCUUAGGGUUUCAUGAACUUAU-5′ |
USP25-DsiRNA-2 | 5′-ACAUGAUGAAGAAUUGAUAUCACAT-3′ 3′-CCUGUACUACUUCUUAACUAUAGUGUA-5′ |
USP25-DsiRNA-3 | 5′-CACUUCUUGUUGGUACCAAAAGGAA-3′ 3′-ACGUGAAGAACAACCAUGGUUUUCCUU-5′ |
HBO1-DsiRNA | 5′-GCUUGAUACCUGGUAUCAUUCUCCA-3′ 3′-CUCGAACUAUGGACCAUAGUAAGAGGU-5′ |