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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Biochim Biophys Acta Gene Regul Mech. 2018 Aug 4;1861(9):773–782. doi: 10.1016/j.bbagrm.2018.08.001

Table 2.

List of PCR primers and DsiRNAs used in the studies.

Primer Sequence
GAPDH forward 5′-GGCATGGACTGTGGTCATGA-3′
GAPDH reverse 5′-TTCACCATGGAGAAGGC -3′
HBO1 forward 5′-AAAGAGACAGGACCTGATTG-3′
HBO1 reverse 5′-GCAACAGAGAAATGACAGAC-3′
USP25 forward 5′-AATGTGATTGATCTCACTGG-3′
USP25 reverse 5′TCATCAGTTATTCCAGTCTCC-3′
IL-1β forward 5′-CTAAACAGATGAAGTGCTCC-3′
IL-1β reverse 5′-GGTCATTCTCCTGGAAGG-3′
IL-6 forward 5′-GCAGAAAAAGCAAAGAATC-3′
IL-6 reverse 5′-CTACATTTGCCGAAGAGC-3′
IL- 10 forward 5′-GCCTTTAATAAGCTCCAAGAG-3′
IL- 10 reverse 5′-ATCTTCATTGTCATGTAGGC-3′
DsiRNA
USP25-DsiRNA-1 5′-AAGAGAAUCCCAAAGUACUUGAATA-3′
3′-AGUUCUCUUAGGGUUUCAUGAACUUAU-5′
USP25-DsiRNA-2 5′-ACAUGAUGAAGAAUUGAUAUCACAT-3′
3′-CCUGUACUACUUCUUAACUAUAGUGUA-5′
USP25-DsiRNA-3 5′-CACUUCUUGUUGGUACCAAAAGGAA-3′
3′-ACGUGAAGAACAACCAUGGUUUUCCUU-5′
HBO1-DsiRNA 5′-GCUUGAUACCUGGUAUCAUUCUCCA-3′
3′-CUCGAACUAUGGACCAUAGUAAGAGGU-5′