Fig 1. CD8+ T cells are resistant to mAb-mediated depletion and more difficult to identify post-depletion.

(A-C) Naïve mice were treated i.p. with a total of 10μg of: anti-CD8α only, anti-CD8β only, or 5μg of both. Splenocytes were isolated and stained the next day. CD8+ T cells were considered CD3+ CD4- B220-. (A) CD8+ T cells as a percentage of lymphocytes and total numbers were calculated. (B) Remaining CD8+ T cell surface bound depleting mAb was determined by staining with Goat anti-Rat IgG. (C) Levels of unbound CD8α and CD8β on surviving CD8+ T cells was determined by staining with the same clones used to deplete. (D) Unmanipulated splenocytes were kept on ice and stained with either anti-CD8α or –β and then stained with the other. Data representative of at least 3 independent experiments. Each point represents the average value of a mouse with 3–5 mice per group.