Table 1. Significance of changes to the Operational Taxonomic Unit (OTU) Richness (a count of the number of OTUs in each sample) & Assemblage (the OTUs making up each sample) during different time periods within the five-year eDNA data including F statistics (F)—PERMANOVA+ [42].

Assay (Number of individual OTUs)	OTU diversity test	Main tests 2010–2014			Main tests Before, During and After
		Month df (30,51)	Season df (15,51)	Year df (4,51)	Heatwave 1 Nov 2010 –April 2011; df (2,54)	Heatwave 2 Nov 2010 –May2012; df (2,54)
Cnidaria	Richness	- F = 2.03	- F = 0.93	- F = 1.16	- F = 1.03	- F = 0.16
(246 OTUs)	Assemblage	- F = 0.91	** F = 1.38	** F = 1.58	*** F = 2.80	*** F = 2.84
Copepod 1	Richness	- F = 4.89	- F = 1.21	** F = 4.63	** F = 6.60	* F = 4.16
(171 OTUs)	Assemblage	* F = 2.15	* F = 1.41	*** F = 1.99	*** F = 3.28	*** F = 3.64
Copepod 2	Richness	- F = 2.76	- F = 0.71	- F = 1.50	- F = 1.67	- F = 0.34
(124 OTUs)	Assemblage	- F = 0.10	- F = 0.22	- F = 1.48	- F = 1.48	* F = 2.01
Copepod 3	Richness	- F = 2.76	- F = 0.71	- F = 1.50	- F = 1.67	- F = 0.34
(342 OTUs)	Assemblage	**F = 2.31	*** F = 1.48	- F = 1.35	**F = 1.94	**F = 2.28
Crustacea	Richness	- F = 0.55	- F = 1.26	- F = 1.96	- F = 2.41	* F = 3.59
(132 OTUs)	Assemblage	- F = 0.86	* F = 1.29	- F = 1.15	- F = 1.08	- F = 1.32
Fish	Richness	- F = 2.38	** F = 3.49	- F = 0.58	- F = 0.09	- F = 0.21
(87 OTUs)	Assemblage	*F = 2.88	*** F = 1.77	- F = 0.79	- F = 1.18	- F = 0.85
Mollusca	Richness	- F = 2.21	- F = 1.27	- F = 0.32	- F = 0.57	- F = 0.47
(345 OTUs)	Assemblage	- F = 1.00	*** F = 1.65	* F = 1.39	*** F = 2.41	*** F = 2.29
Universal	Richness	- F = 0.52	- F = 0.96	- F = 2.02	- F = 0.40	- F = 1.78
(97 OTUs)	Assemblage	- F = 0.96	* F = 1.34	- F = 1.45	** F = 2.08	*** F = 2.45

Where ***is P ≤ 0.001, **is P ≤ 0.01, *is P≤ 0.05 & – is no significant change