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. 2019 Feb 8;15(2):e1007579. doi: 10.1371/journal.ppat.1007579

Fig 1. B. anthracis spore surface protein BclA mediated CFH binding to spores.

Fig 1

Spores were incubated with purified human CFH in PBS buffer containing D-alanine. Spore-bound CFH was determined by flow cytometry (A), solid phase binding assay (B) and Western blot (C). Flow cytometry results were combined from at least three independent experiments. Solid phase binding assay results were combined from two independent experiments, each with duplicate wells. Western blots shown were representative of at least three independently performed experiments. (D) Recombinant BclA protein (rBclA) bound to immobilized human CFH in a concentration-dependent manner. Results were combined from three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001; t test.