Table 3. Analysis of the sensitivity of bacterial, fungal and viral target detection on the Axiom Microbiome Array.
The genomic DNA was spiked into soil DNA background. The average number of probes detected was an average of two replicates with standard deviation.
| Target spiked | Genome copy number | Target detected as | Average # of probes detected /expecteda |
|---|---|---|---|
| Aspergillus fumigatus | 10 | Not detected | NA |
| 100 | A. fumigatus Af10 | 55±1 /168 | |
| 1000 | A. fumigatus Af10 | 81±0 /168 | |
| 10000 | A. fumigatus Af10 | 83±0 /168 | |
| Shigella flexneri | 10 | Not detected | NA |
| 100 | S. flexneri 2930–71 draft (50 frags) | 2185±42 /7398 | |
| 1000 | S. flexneri II:(3)4 7(8) draft (196 frags) | 3781±125 /8009 | |
| 10000 | S. flexneri II:(3)4 7(8) draft (196 frags) | 4027±88 /8009 | |
| Vaccinia lister | 100 | Not detected | NA |
| 1000 | Vaccinia virus strain DUKE | 78±4 /293 | |
| 10000 | Vaccinia virus | 148±1 /286 |
a The number of probes detected vs expected at different concentrations could be different if a different target strain was predicted by the array.