Table 5. Axiom Microbiome Array results of ATCC 20 Strain Staggered Mix Genomic Material (ATCC MSA-1003).
Organisms in the sample mix | Percentage | Copies/ng | Correct species Detected? | Axiom Microbiome Array target detection (chromosome and plasmid) |
---|---|---|---|---|
Streptococcus mutans strain UA159 (ATCC 700610) | 18.00 | 82,149 | Yes | Streptococcus mutans UA159 chromosome |
Porphyromonas gingivalis (ATCC 33277) | 18.00 | 71,266 | Yes | Porphyromonas gingivalis ATCC 33277 |
Staphylococcus epidermidis (ATCC 12228) | 18.00 | 69,485 | Yes | Staphylococcus epidermidis ATCC 12228 chromosome, plasmid pSE-12228-03, -04, -05, -06 |
Rhodobacter sphaeroides (ATCC 17029) | 18.00 | 40,378 | Yes | Rhodobacter sphaeroides ATCC 17029 chromosome 2, pRSPH01, Rhodobacter sphaeroides WS8N draft (4 frags) |
Escherichia coli Strain MG1655 (ATCC 700926) | 18.00 | 35,482 | Yes | Escherichia coli str. K12 substr. MG1655 |
Streptococcus agalactiae strain 2603 V/R (ATCC BAA-611) | 1.80 | 7,546 | Yes | Streptococcus agalactiae 2603V/R chromosome |
Staphylococcus aureus strain FPR3757 (ATCC BAA-1556) | 1.80 | 5,956 | Yes | Staphylococcus aureus W89268 draft (24 frags)a |
Bacillus cereus (ATCC 10987) | 1.80 | 3,082 | Yes | Bacillus cereus ATCC 10987, plasmid pBc10987 |
Clostridium beijerinckii strain SA-1 (ATCC 35702) | 1.80 | 2,779 | Yesb | Clostridium beijerinckii NCIMB 8052 chromosomec |
Pseudomonas aeruginosa strain R. Hugh 813 (ATCC 9027) | 1.80 | 2,647 | Yes | Pseudomonas aeruginosa PA7 chromosome |
Helicobacter pylori strain 26695 (ATCC 700392) | 0.18 | 999 | Yes | Helicobacter pylori 26695–1 DNA |
Lactobacillus gasseri (ATCC 33323) | 0.18 | 882 | Yes | Lactobacillus gasseri ATCC 33323 chromosome |
Neisseria meningitidis strain MC58 (ATCC BAA-335) | 0.18 | 735 | Yes | Neisseria meningitidis 9757 draft (32 frags) |
Propionibacterium acnes (ATCC 11828) | 0.18 | 651 | Yes | Propionibacterium acnes ATCC 11828 chromosomed |
Acinetobacter baumannii (ATCC 17978) | 0.18 | 419 | Yes | Acinetobacter baumannii ATCC 17978 chromosome, plasmid pAB2 |
Bifidobacterium adolescentis (ATCC 15703) | 0.02 | 89 | No | NA |
Actinomyces odontolyticus (ATCC 17982) | 0.02 | 79 | No | NA |
Deinococcus radiodurans (ATCC BAA-816) | 0.02 | 70 | Yes | Deinococcus radiodurans R1 plasmid CP1, MP1 |
Enterococcus faecalis (ATCC 47077) | 0.02 | 61 | No | NA |
Bacteroides vulgatus (ATCC 8482) | 0.02 | 36 | Yes | Bacteroides vulgatus ATCC 8482 chromosome |
a In 1 out of 2 replicates, this was detected as Staphylococcus aureus M79686 draft (25 frags)
b Detected in 1 out of 2 replicates.
c Clostridium beijerinckii SA-1 was derived by directed evolution from C. beijerinckii NCIMB8052.
d In 1 out of 2 replicates, this was detected as Propionibacterium acnes J139 draft (7 frags).