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. 2019 Feb 8;14(2):e0201332. doi: 10.1371/journal.pone.0201332

Fig 4. Gating of PNA-FISH hybridized BC samples.

Fig 4

Bacteria-specific events were separated from the background noise of lysed blood based on a difference between the PNA probe-specific signal and autofluorescence produced by blood (i). This was followed by identification of single cells on the FSC-H/FSC-A scatter plot within the bacterial population (ii). Probe specific signal was gated as AlexaFluor 488 + on the histogram (iii), and all events within this gate were backgated and shown on the FSC-H/SSC-H plot as dPOI (iv).