Table 4. Bacterial enumeration using PNA-FISH and plate counts at the time of detection.
Bacterial species | BC incubation in the BacTEC system (hours) | PNA-FISH-AFC (events/mL±SD) |
Plate counts (CFU/mL ± SD) |
---|---|---|---|
Escherichia coli | 5 | 0.17 × 104 ± 0.11 | 0.21 × 104 ± 0.27 |
6 | 0.40 × 104 ± 0.26 | 0.57 × 104 ± 0.72 | |
7 | 0.28 × 104 ± 2.72 | 2.94 × 104 ± 1.05 | |
Klebsiella pneumoniae | 5 | 0.25 × 104 ± 0.40 | 0.44 × 104 ± 0.62 |
6 | 0.64 × 104 ± 0.79 | 1.41 × 104 ± 1.44 | |
7 | 5.29 × 104 ± 6.24 | 9.24 ×104 ± 12.2 | |
Pseudomonas aeruginosa | 10 | 1.09 × 104 ± 0.80 | 1.31 × 104 ± 1.38 |
11 | 2.36 × 104 ± 1.81 | 3.10 × 104 ± 3.80 | |
12 | 7.52 × 104 ± 8.31 | 12.1 × 104 ± 18.2 |