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. Author manuscript; available in PMC: 2019 Feb 8.
Published in final edited form as: Mol Biol Rep. 2018 Jul 21;45(5):1551–1556. doi: 10.1007/s11033-018-4251-3

Figure-1. Primers designed and genetic construct assembly.

Figure-1.

(A-B) Forward primer-containing T7 polymerase promoter, spacer sequences and the Kozak sequence (CCACC) was synthesized. In the reverse primer a tag of 6-Histidine and a poly Adenine tail sequence for RNA product stability were added. (C) PCR product amplified with these primers was analyzed by 1.5% agarose gel electrophoresis resulting in a ~390-bp product, which include ~300-bp FhSAP2 sequence + 39-bp T7 forward + 51-bp T7 reverse.