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. Author manuscript; available in PMC: 2019 Feb 8.
Published in final edited form as: Mol Biol Rep. 2018 Jul 21;45(5):1551–1556. doi: 10.1007/s11033-018-4251-3

Figure-2. Cell free expression of F. hepatica saposin-like protein-2.

Figure-2.

Target gene was PCR amplified from the cloned vector and used as the template for the cell-free protein synthesis. (A) Two polypeptides of ~12–14kDa and ~29kDa corresponding to monomeric and oligomeric forms of FhSAP2, respectively were revealed in all tubes. (B) The content of all tubes was pooled. The His-tagged protein was purified by Ni-affinity chromatography by elution with a buffer containing 20mM sodium phosphate containing 0.5 M NaCl + 500mM imidazole, pH 7.4 (dashed line).