Figure 4.

TUG1 promotes tumorigenesis of PC cells in vivo.

Notes: (A) Empty vector or sh-TUG1 was transfected into BxPC-3 cells, which were subsequently injected individually into nude mice (n=7). (B) Tumor volumes were calculated every 2 days after injection. The mean tumor volumes are indicated by points and the bars indicate SD (n=7). (C) Tumor weights are represented as the mean tumor weights ± SD. (D) qRT-PCR analysis was performed to determine the average expression levels of TUG1 in the xenograft tumors (n=7). (E) The tumor sections were examined using H&E staining and immunohistochemical staining with antibodies against Ki-67. The error bars indicate the mean ± standard error. *P<0.05 and **P<0.01.

Abbreviations: PC, pancreatic cancer; TUG1, taurine upregulated 1; qRT, quantitative real-time.