Figure 8.

Schematic distribution of characteristic motifs of Prs polypeptides. The Prs3 polypeptide consists of 320 aa, and the position of ₂₈₄KKCPK₂₈₈ is indicated by a hatched box. The positions of the diagnostic features of a Prs polypeptide are indicated on the open bar, and their coordinates below the open bar were obtained by comparing the sequence of B. subtilis Prs lacking its initial methionine with that of S. cerevisiae Prs3. The insertion of Prs1, NHR1–1, is indicated by a triangle above the open bar. The two insertions in Prs5, NHR5–1 and NHR5–2, are indicated by triangles below the open bar. The coordinates of the insertions refer to their positions in the sequences of Prs1 and Prs5, respectively. The numbering of their insertion points corresponds to the sequence of the Prs3 polypeptide. All sequence comparisons were performed using the CLUSTALW multiple alignment tool (weight matrix = Gonnet) available in Expasy (NPS@: Network Protein Sequence Analysis (Combet et al.2000).