Table 1.

Y2H interactions of Prs polypeptides with Nuf2 in PJ69–4A.

Gal4BD	Gal4AD	β-Galactosidase filter assay	Histidine prototrophy	Adenine prototrophy	β-Galactosidase activity
–	–	–	–	–	2.8 ± 0.3
Snf4	Snf1	+	+	+	11.4 ± 0.7
Prs5	Prs4	+	+	+	11.1 ± 0.1
Nuf2	Prs1	+	+	+	25.7 ± 1.3
Nuf2	Prs2	+	+	+	39.9 ± 0.5
Nuf2	Prs3	+	+	+	56.3 ± 0.6
Nuf2	Prs4	+	–	–	1.7 ± 0.1
Nuf2	Prs5	+	+	+	26 ± 0.3

The β-galactosidase activity (nmole ONPG min⁻¹ mg⁻¹) represented the mean ± SD from at least three independent experiments.