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. Author manuscript; available in PMC: 2020 Feb 7.
Published in final edited form as: Cell. 2019 Jan 10;176(4):805–815.e8. doi: 10.1016/j.cell.2018.12.001

Figure 2. Palmitoylation status of importin α modulates both spindle and nuclear size.

Figure 2.

(A) Fluorescence images and quantification of spindle lengths in metaphase-arrested egg extract reactions containing 1 μM recombinant wild-type or NP mutant importin α in the presence of DMSO or 10 μM palmostatin. Importin α NP reverted the decrease in spindle length caused by importin α hyperpalmitoylation. Mean ± SD, 64 spindles from 3 extracts. ** = p < 0.0005.

(B) Fluorescence images of DNA staining and quantification of nuclear diameter in interphase egg extract reactions containing 1 μM recombinant wild-type or NP mutant importin α in the presence of DMSO or 10 μM palmostatin. Importin α NP reverted the decrease in nuclear diameter caused by importin hyperpalmitoylation. Mean ± SD, 78 nuclei from 3 extracts. ** = p < 0.0005.

(C) Fluorescence images and quantification of spindle lengths in metaphase-arrested egg extract reactions containing DMSO or 1 μM Wnt-C59, which reduced importin α palmitoylation and increased spindle length. Mean ± SD, 67 spindles from 3 extracts. * = p < 0.005.

(D) Fluorescence images of DNA staining and quantification of nuclear diameter in interphase egg extract reactions containing 1 μM Wnt-C59, which reduced importin α palmitoylation and decreased nuclear size. Mean ± SD, 319 nuclei from 3 extracts. ** = p < 0.0005.