(A) Fluorescence images and quantification of spindle lengths in metaphase-arrested egg extract reactions containing 1 μM recombinant wild-type or NP mutant importin α in the presence of DMSO or 10 μM palmostatin. Importin α NP reverted the decrease in spindle length caused by importin α hyperpalmitoylation. Mean ± SD, 64 spindles from 3 extracts. ** = p < 0.0005.
(B) Fluorescence images of DNA staining and quantification of nuclear diameter in interphase egg extract reactions containing 1 μM recombinant wild-type or NP mutant importin α in the presence of DMSO or 10 μM palmostatin. Importin α NP reverted the decrease in nuclear diameter caused by importin hyperpalmitoylation. Mean ± SD, 78 nuclei from 3 extracts. ** = p < 0.0005.
(C) Fluorescence images and quantification of spindle lengths in metaphase-arrested egg extract reactions containing DMSO or 1 μM Wnt-C59, which reduced importin α palmitoylation and increased spindle length. Mean ± SD, 67 spindles from 3 extracts. * = p < 0.005.
(D) Fluorescence images of DNA staining and quantification of nuclear diameter in interphase egg extract reactions containing 1 μM Wnt-C59, which reduced importin α palmitoylation and decreased nuclear size. Mean ± SD, 319 nuclei from 3 extracts. ** = p < 0.0005.