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. Author manuscript; available in PMC: 2020 Feb 7.
Published in final edited form as: Cell. 2019 Jan 10;176(4):805–815.e8. doi: 10.1016/j.cell.2018.12.001

Figure 7. Palmitoylation modulates importin α localization, nuclear and spindle size in human cells.

Figure 7.

(A) Fluorescence images of importin α localization and quantification of spindle lengths in RPE-1 cells treated with either DMSO, 50 μM palmostatin or 10 μM Wnt-C59 for 12 hours. Palmostatin treatment increased importin α localization to the plasma membrane and decreased spindle size while Wnt-C59 treatment had the opposite effect. Mean ± SD, 199 cells from 3 experiments. ** = p < 0.0005.

(B) Fluorescence images of DNA and importin α staining and quantification of nuclear area of RPE-1 cells treated with either DMSO, 50 μM palmostatin or 10 μM Wnt-C59 for 12 hours. Palmostatin treatment increased importin α localization to the plasma membrane and decreased nuclear size while Wnt-C59 treatment had the opposite effect. Mean ± SD, 213 cells from 3 experiments. ** = p < 0.0005.

(C) Fluorescence images of metaphase spindles and quantification of spindle lengths in HCT 293 cells 3 days after transfection of either scrambled siRNA or siRNAs targeted to LYPLA1 and PORCN. LYPLA1 knockdown decreased spindle size while PORCN knockdown had the opposite effect. Mean ± SD, 168 cells from 3 experiments. *** = p < 0.0005.

(D) Fluorescence images of metaphase spindles and quantification of nuclear area in HCT 293 cells 3 days after transfection of either scrambled siRNA or siRNAs targeted to LYPLA1 and PORCN. LYPLA1 knockdown decreased nuclear area while PORCN knockdown had the opposite effect. Mean ± SD, 87 cells from 3 experiments. * = p < 0.05.