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. Author manuscript; available in PMC: 2020 Feb 7.
Published in final edited form as: Cell. 2019 Jan 10;176(4):805–815.e8. doi: 10.1016/j.cell.2018.12.001
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rabbit polyclonal anti-importin α Levy and Heald, 2010 N/A
Rabbit polyclonal anti-kif2a Abcam ab37005
Rabbit polyclonal anti-TPX2 This paper N/A
Monoclonal anti-GFP Clontech Cat#632380
Rabbit anti-XCTK2 Walczak et al, 1997 N/A
Rabbit anti-Xkid Funabiki et al, 2000 N/A
Goat anti-LYPLA1 Abcam ab77640
Monoclonal anti-GFP Clontech Cat#632380
Rabbit anti-PORCN Abcam ab105543
Chemicals, Peptides, and Recombinant Proteins
Palmostatin B Millipore Sigma Cat#178501–5MG
Wnt C-59 Cayman Chemical Cat#1243243–89-1
Quinalizarin Sigma-Aldrich Q2763
Experimental Models: Cell Lines
Human: hTERT RPE-1 UC Berkeley Cell Culture Facility N/A
Human: HCT 116 UC Berkeley Cell Culture Facility N/A
Human: HEK-293 UC Berkeley Cell Culture Facility N/A
Experimental Models: Organisms/Strains
Xenopus laevis NXR
Oligonucleotides
siRNA: LYPLA1 Origene Cat#SR307076
siRNA:PORCN Origene Cat#SR324902
Recombinant DNA
Plasmid: Importin1α GFP This paper N/A
Plasmid: Importin α RFP This paper N/A
Plasmid: Importin α GFP NP This paper N/A
Plasmid: Importin α GFP E This paper N/A
Plasmid: Pet20 (a,b,c) EMD Millipore Cat#69739