REAGENT or RESOURCE |
SOURCE |
IDENTIFIER |
Antibodies |
Rabbit polyclonal anti-importin α |
Levy and Heald, 2010 |
N/A |
Rabbit polyclonal anti-kif2a |
Abcam |
ab37005 |
Rabbit polyclonal anti-TPX2 |
This paper |
N/A |
Monoclonal anti-GFP |
Clontech |
Cat#632380 |
Rabbit anti-XCTK2 |
Walczak et al, 1997 |
N/A |
Rabbit anti-Xkid |
Funabiki et al, 2000 |
N/A |
Goat anti-LYPLA1 |
Abcam |
ab77640 |
Monoclonal anti-GFP |
Clontech |
Cat#632380 |
Rabbit anti-PORCN |
Abcam |
ab105543 |
Chemicals, Peptides, and Recombinant Proteins |
Palmostatin B |
Millipore Sigma |
Cat#178501–5MG |
Wnt C-59 |
Cayman Chemical |
Cat#1243243–89-1
|
Quinalizarin |
Sigma-Aldrich |
Q2763 |
Experimental Models: Cell Lines |
Human: hTERT RPE-1 |
UC Berkeley Cell Culture Facility |
N/A |
Human: HCT 116 |
UC Berkeley Cell Culture Facility |
N/A |
Human: HEK-293 |
UC Berkeley Cell Culture Facility |
N/A |
Experimental Models: Organisms/Strains |
Xenopus laevis |
NXR |
|
Oligonucleotides |
siRNA: LYPLA1 |
Origene |
Cat#SR307076 |
siRNA:PORCN |
Origene |
Cat#SR324902 |
Recombinant DNA |
Plasmid: Importin1α GFP |
This paper |
N/A |
Plasmid: Importin α RFP |
This paper |
N/A |
Plasmid: Importin α GFP NP |
This paper |
N/A |
Plasmid: Importin α GFP E |
This paper |
N/A |
Plasmid: Pet20 (a,b,c) |
EMD Millipore |
Cat#69739 |