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. Author manuscript; available in PMC: 2019 Mar 3.
Published in final edited form as: Dev Biol. 2018 Dec 27;446(2):168–179. doi: 10.1016/j.ydbio.2018.12.023

Figure 2. H3K27ac+ gonad-specific NDRs are distal enhancers that neighbor granulosa-and Sertoli-promoting genes.

Figure 2.

A-C) Graphs showing the average number of NDRs associated to the nearest TSS as determined by HOMER. Based on their cell-type specificity, NDRs were classified as ubiquitous (A), gonad-specific (B) or H3K27ac+ gonad-specific (C). NDRs were associated to the nearest TSS of genes that become granulosa cell-specific (pink, 823 total genes) and Sertoli cell-specific (blue, 1026 total genes). Granulosa-specific and Sertoli-specific genes were obtained from (Jameson et al., 2012b). Error bars represent the standard error of the mean (SEM) and ** represents p<0.001 as determined by a student’s t test. D) Ubiquitous, gonad-specific and gonad-specific H3K27ac-positive NDRs were subcategorized based on their genomic location (promoter, grey; exonic, orange; intronic, green; intergenic, purple). E) Ubiquitous, gonad-specific and gonad-specific H3K27ac-positive NDRs were subcategorized based on their 5’ (left) and 3’ (right) distance to the associated TSS (TSS, black middle line; 0–1kb, light grey; 1–3kb, purple; 3–5kb, blue; 5–10kb, green; 10–100kb, medium grey; >100kb, dark grey).